Multicellular Quadruple Colorectal Cancer Spheroids as an In Vitro Tool for Antiangiogenic Potential Evaluation of Nanoparticles

The encapsulation of bevacizumab (BVZ), angiogenesis inhibitor antibody, into nanocarriers is explored aiming at enhancing its efficacy in colorectal cancer (CRC) treatment while eliminating potential side effects. Still, the translation such nanomedicines clinics not straightforward owing to their preclinical screening simplistic models, that do mimic complexity and heterogeneity CRC microenvironment. Herein, development a multicellular spheroid as an advanced model capable antiangiogenic novel proposed. For that, quadruple co-culture established through combination HCT116 cells, human pulmonary microvascular endothelial cell (HPMEC), fibroblasts, macrophages. It demonstrated developed displays intrinsic features, organization expression tumor microenvironment, extracellular matrix, formation necrotic core. Moreover, shown be composed mostly (93%), followed by hypoxia-inducible factor (3%), HPMEC macrophages (1%). Gellan gum/chitosan nanoparticles encapsulating BVZ exhibit superior properties when compared free BVZ. Overall, can further promising approach treatment.